Classical and Basal Cell Culture Media
NEOGEN Tetrathionate Broth Base, 500 g
Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller’s broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-broth-base
NEOGEN Columbia CNA Agar, 500 g
Columbia CNA Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Columbia CNA Agar is not intended for use in the diagnosis of disease or other conditions in humans.Ellner et al. described Columbia CNA Agar as a variation of Columbia Blood Agar Base that is selective for Gram-positive cocci. Colistin and Nalidixic Acid are added to the formula, selecting for Gram-positive organisms and fungi by suppressing Gram-negative bacteria.For more information visit https://www.neogen.com/solutions/Microbiology/columbia-cna-agar
NEOGEN Brilliant Green Agar, 500 g
Brilliant Green Agar is used for the selective isolation of Salmonella spp. in a laboratory setting. Brilliant Green Agar is not intended for use in the diagnosis of disease or other conditions in humans.Kristensen, Lester, and Jurgens first described the use of Brilliant Green Agar as a primary plating medium for the isolation of Salmonella. The report described the medium as useful for the differentiation of “paratyphoid B” from other intestinal gram-negative bacilli. Kaufmann modified their formula and used Brilliant Green Agar in addition to Tetrathionate Broth for the isolation of Salmonella from stool specimens. The outstanding selectivity of this medium permits use of moderately heavy inocula, which should be evenly distributed over the surface. Brilliant Green Agar is used in the microbial limits test. Brilliant Green Agar supplemented with novobiocin is used in food testing.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-ncm0283
NEOGEN Brain Heart Infusion Broth, 500 g
Brain Heart Infusion Broth is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans. A rich isotonic infusion medium with tryptose (a mixture of meat and milk peptones) providing a wide range of substrates. A low concentration of glucose is used to stimulate early growth. The medium is lightly buffered to prevent the early death of some species due to acid production. Organisms which produce significant amounts of acid may well overwhelm the buffering system and auto-sterilize. The medium is suitable for use as a blood culture medium or as an enrichment broth for fastidious organisms. Formula:Brain Heart Infusion Solids (Porcine) - 17.5 g/L*Tryptose - 10.0 g/LGlucose - 2.0 g/LSodium Chloride - 5.0 g/LDisodium Hydrogen Phosphate - 2.5 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/brain-heart-infusion-broth
NEOGEN Cystine Lactose Electrolyte Deficient (CLED) Agar, 500 g
Cystine Lactose Electrolyte Deficient (CLED) Agar is used for the differentiation and enumeration of microorganisms in urine. A medium for urine culture where the absence of electrolytes inhibits the swarming of Proteus spp. Cystine is added for the benefit of those organisms which have a specific cystine requirement. Differentiation of lactose and non-lactose fermenters is achieved using bromothymol blue as pH indicator. This medium supports the growth of Streptococcus pyogenes and most other fastidious organisms that do not require blood. Formula:Balanced Peptone - 4.0 g/LBeef Extract - 3.0 g/LTryptone - 4.0 g/LLactose - 10.0 g/LL-Cystine - 0.128 g/LBromothymol Blue - 0.02 g/LAgar - 15.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/cystine-lactose-electrolyte-deficient-agar
NEOGEN XLT4 Agar, 500 g
XLT4 Agar is used with Tergitol 4 for the isolation of non-typhi Salmonella spp and is not intended for use in the diagnosis of disease or other conditions in humans. XLT4 Agar is a selective differential isolation medium for the specific detection of Salmonella spp. from environmental and food samples. Due to its highly selective nature, XLT4 Agar is particularly effective when used with samples where overgrowth of contaminating flora is expected, for example, fecally contaminated agricultural samples. Developed to perform as per Miller & Tate in 1990, this medium was found to improve the recovery of non-typhi Salmonella from chicken and farm environmental samples. Dusch and Altwegg further established the application of XLT4 Agar to salmonellae detection in clinical samples, with the notable exceptions of Salmonella typhi and Salmonella paratyphi.For more information visit https://www.neogen.com/solutions/Microbiology/xlt4-agar
NEOGEN MacConkey Broth, 500 g
MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonized USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar. For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-broth
NEOGEN Nutrient Broth No. 2, 500 g
Nutrient Both No. 2 is used for the cultivation of fastidious bacteria, and forms the base for Preston Broth according to ISO 10272-1:2017 for the cultivation of Campylobacter spp. This media is not intended for use in the diagnosis of disease or other conditions in humans. This broth can also be used as the suspending medium for cooked meat granules for the cultivation of anaerobic organisms.For more information visit https://www.neogen.com/solutions/Microbiology/nutrient-broth-number-2
NEOGEN Lactobacilli MRS Broth, 500 g
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-broth
NEOGEN EC Medium with MUG, 500 g
EC Medium w/ MUG is used for the fluorogenic detection of Escherichia coli in a laboratory setting. EC Medium w/ MUG is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria is inhibited by the bile salts, while growth of E. coli is enhanced by its presence. EC Medium w/ MUG is the same formula as EC Medium, with the addition of 4-methylumbelliferyl-ß-D-glucuronide. Feng and Hartman developed a rapid assay for E. coli by incorporating 4-methylumbelliferyl-ß-D-glucuronide (MUG) into Lauryl Tryptose Broth at a final concentration of 100 ɥg/mL.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-with-mug
NEOGEN Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti, 500 g
Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. This medium is not intended for use in the diagnosis of disease or other conditions in humans.Lithium chloride in the base medium and supplementary antimicrobial compounds Ceftazidime, Polymyxin, Nalidixic acid and Cycloheximide provide the medium’s selectivity. Chromogenic activity is as a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. and to a few strains of Enterococci and Bacilli.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-listeria-chromogenic-agar-ottaviani-and-agosti
NEOGEN Tryptic Soy Broth (Soybean-Casein Digest Broth), 500 g
Tryptic Soy Broth (Soybean-Casein Digest Broth) is used for the cultivation of a wide variety of microorganisms. Tryptic Soy Broth conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean-Casein Digest broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth
NEOGEN Brilliant Green Bile 2% Broth, 500 g
Brilliant Green Bile Broth 2% is used for the detection of coliform bacteria in water, food, and dairy products in a laboratory setting. Brilliant Green Bile Broth 2% is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products and other materials. Brilliant Green Bile Broth 2% is used to confirm a positive presumptive test result.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-bile-2-percent-broth
Lonza Walkersville Rodent and Monkey Cryopreserved Hepatocyte Thawing Medium
Rodent and Monkey Cryopreserved Hepatocyte Thawing Medium is supplied in ready to use in 50mL conical tubes. Medium has been optimized specifically for monkey, mouse, and rat primary hepatocytes to ensure high viability and yield is maintained during reanimation from cryopreservation.Benefits:Optimized for thawing rodent and monkey hepatocytesApplications:ADMEToxContent:50mL conical tube
Accurate Chemical Nycodenz Density Gradient Medium, Powder, Off-white, 500 g
Nycodenz 500GM is a universal, non-ionic, and non-toxic density gradient medium designed for the high-resolution separation of biological particles. It comes as an off-white powder that forms stable low-osmolarity solutions. This product is ideal for isolating various cellular components without affecting their viability. Nycodenz has minimal interference with biochemical assays, making it an excellent choice for researchers seeking reliable separation methods.
- Form: Powder
- Color: Off-white
- Chemical Name: Iohexol
- Molecular Weight: 821 Da
- Density (Solid): 2.1 g/ml
- Maximum Solution Density: 1.426 g/ml (at 80% w/v)
- Solubility: Water, formamide, dimethyl formamide
- Stability: Up to 5 years
- Packaging Size: 500 g